Chai’s Sports Gene educational kit is designed to provide high school and college students with an introductory exposure to the real-time PCR process for DNA detection. Students will gain hands-on experience to molecular diagnostic procedures commonly used in the real world by life scientists and industrial microbiologists for various applications (molecular diagnostics, genetics, water quality, food testing, forensics and more). Specifically, students will run a real-time PCR experiment to detect for the presence of ACTN3 variant ( ACTN3 R577X SNP -single nucleotide polymorphism), which is found to be associated with power athletic performance, especially among male and female track and field athletes. The kit is optimized for the Open qPCR instrument.
The kit allows a hands-on step-by-step approach to learning. Students conduct sophisticated DNA target detection procedures, similar to ones performed in the real-world, that employ the real-Time PCR process.
- Extract and amplify DNA sample collected from buccal swabs
- Perform realistic diagnostic procedures
- Use real-time PCR and melt curve analysis
Students determine the presence or absence of the sports Gene from their buccal swab samples and answer the following questions:
- Did we successfully extract DNA?
- Did our PCR work as expected?
- What is my ACTN3 genotype?
For classroom use and the recommended curriculum, please email firstname.lastname@example.org to connect with an Application Specialist.
The kit includes all needed reagents and consumables for 50 reactions. The user will need to provide adjustable micropipettes (200 uL and 20 uL volumes) with the respective filter tips.
|Extraction Buffer A||25 mL|
|Reconstitution Buffer “C”||0.05 mL|
|ACTN WT (control)||Lyophilized|
|ACTN Mutant (control)||Lyophilized|
|PCR Master Mix + Hot Start DRY||Lyophilized|
|Reconstitution Buffer||0.7 mL|
|Chai Green 20X Dye||1 mL|
|1.5 mL tubes||60|
|8-strip PCR Strip Tubes||15|
|8-strip PCR Strip Caps||15|