One-Step DNA/RNA Extraction Buffer 1X
- Simple extraction of PCR-ready DNA and RNA without centrifugation
- Lyses cells in minutes at ambient temperature, without mechanical agitation
- Binds and inactivates inhibitors commonly found in environmental samples
- Stabilizes RNA for 24 hours at 25 ºC in most samples
Lyse and extract DNA/RNA from:
- Bacteria + protozoa
- Animal cells
Buffer is safe to use; all reagents are non-toxic.
Stabilizes DNA and RNA
DNA is a fairly robust molecule, but RNA traditionally requires storage at -20 or -80 ºC. Chai's One-Step DNA/RNA Extraction Buffer stabilizes RNA, allowing for handling at 25 ºC for 24 hours in most samples. RNA can be stored for days at -20 ºC, or at -80 ºC for long-term storage.
Buffer binds and inactivates many PCR inhibitors found in environmental samples and crude extracts. The resulting DNA and RNA is suitable for PCR or RT-PCR without further purification.
|Lysis Temperature||20 – 25 ºC|
|Sample Types||Bacteria, animal cells, viral|
|DNA Stability Post-Extraction||Weeks at 25 ºC, indefinite at -20 ºC|
|RNA Stability Post-Extraction||24 hours at 25 ºC, days at -20 ºC|
Chai's One-Step DNA/RNA Extraction Buffer 1X may be used license-free for commercial or diagnostic applications, and is available in bulk quantities for OEM use.