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PIKA 4e L. acetotolerans PCR Test Kit

PIKA 4everyone L. acetotolerans Beer Spoiler Test Kit


48 tests/kit


  • Test beer samples for Lactobacillus acetotolerans in two hours
  • Real-Time PCR test delivers quantitative, objective results
  • Automatic analysis with Open qPCR allows anyone to run tests


Lactobacillus acetotolerans spoils beer by the production of lactic acid. It often starts growth after a lengthy inactive phase in finished or aging beer, but can proliferate fast, causing an extreme drop in pH. L. acetotolerans is sensitive to oxygen and often shows minimal growth, making it difficult to detect by enrichment alone. Its slow growth pattern makes it a spoiler of particular importance in barrel-aged beers.

The PIKA 4e L. acetotolerans test kit specifically detects and quantifies L. acetotolerans, excluding all other Lactobacillus species. Includes all reagents needed to extract and amplify DNA using Real-Time PCR. 48 reactions/test kit.


Sensitivity10 CFU/mL (direct PCR)
1 cell/sample (enriched)
PCR Time75 minutes
Compatible SamplesBeer, yeast slurry, colonies, environmental
ControlsPositive, negative, internal inhibition
Automatic AnalysisSupported with Open qPCR
Shipping TemperatureAmbient
Storage Temperature2 – 8 °C
ManufacturerPIKA Weihenstephan
Manufacturer Part #2401-52

Easy Workflow

Brewing Quality Solution Workflow
  • 20 minutes hands-on time.
  • Samples may be directly tested for 2 hour results.
  • Pre-enrichment with PCR-grade FastOrange increases sensitivity & degrades dead cells.
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