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Lactobacillus and Pediococcus can spoil beer by the production of lactic acid. However of the over 200 species of these genera, only thirteen species have been proven to spoil beer.

The PIKA 4e Real Beer Spoiler test kit screens for only those thirteen specific species, preventing the false positives common in less-sensitive kits, or kits relying on hops resistance genes. Read more about the Real Beer Spoilers of Lactobacillus and Pediococcus.

Spoilers are quantitatively detected using probe-based Real-Time PCR. Includes all reagents needed to extract and amplify DNA. 48 reactions/test kit.

Detected Species

L. acetotolerans
L. backii
L. brevis
L. casei
L. collinoides
L. coryniformis
L. lindneri
L. parabuchneri (“frigidus”)
L. perolens
L. plantarum
L. rossiae
P. damnosus
P. inopinatus


Sensitivity10 CFU/mL (direct PCR)
1 cell/sample (enriched)
PCR Time75 minutes
Compatible SamplesBeer, yeast slurry, colonies, environmental
ControlsPositive, negative, internal inhibition
Automatic AnalysisSupported with Open qPCR
Shipping TemperatureAmbient
Storage Temperature2 – 8 °C
ManufacturerPIKA Weihenstephan
Manufacturer Part #2401-38

Easy Workflow

Brewing Quality Solution Workflow
  • 20 minutes hands-on time.
  • Samples may be directly tested for 2 hour results.
  • Pre-enrichment with PCR-grade FastOrange increases sensitivity & degrades dead cells.
The Real Beer Spoilers of Lactobacillus & Pediococcus


Product Manual Download
Material Safety Data Sheet (MSDS) Download