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PIKA 4e Megasphaera PCR Test Kit

PIKA 4everyone Megasphaera Beer Spoiler PCR Test Kit


48 tests/kit


  • Test beer samples for Megasphaera spoilage bacteria in two hours
  • Real-Time PCR test delivers quantitative, objective results
  • Automatic analysis with Open qPCR allows anyone to run tests


Megasphaera spoils beer by producing a putrid, fecal smell. Because it is an anaerobic bacteria extremeley sensitive to oxygen, it is difficult to detect by enrichment alone.

The PIKA 4e Megasphaera test kit screens for and quantifies all Megasphaera species. Includes all reagents needed to extract and amplify DNA using Real-Time PCR. 48 reactions/test kit.

Detected Species

M. cerevisiae
M. elsdenii
M. micronuciformis
M. paucivorans


Sensitivity10 CFU/mL (direct PCR)
1 cell/sample (enriched)
PCR Time75 minutes
Compatible SamplesBeer, yeast slurry, colonies, environmental
ControlsPositive, negative, internal inhibition
Automatic AnalysisSupported with Open qPCR
Shipping TemperatureAmbient
Storage Temperature2 – 8 °C
ManufacturerPIKA Weihenstephan
Manufacturer Part #2401-41

Easy Workflow

Brewing Quality Solution Workflow
  • 20 minutes hands-on time.
  • Samples may be directly tested for 2 hour results.
  • Pre-enrichment with PCR-grade FastOrange increases sensitivity & degrades dead cells.
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Chai + PIKA Brewing Quality Solutions