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Megasphaera spoils beer by producing a putrid, fecal smell. Because it is an anaerobic bacteria extremeley sensitive to oxygen, it is difficult to detect by enrichment alone.

The PIKA 4e Megasphaera test kit screens for and quantifies all Megasphaera species. Includes all reagents needed to extract and amplify DNA using Real-Time PCR. 48 reactions/test kit.

Detected Species

M. cerevisiae
M. elsdenii
M. micronuciformis
M. paucivorans


Sensitivity10 CFU/mL (direct PCR)
1 cell/sample (enriched)
PCR Time75 minutes
Compatible SamplesBeer, yeast slurry, colonies, environmental
ControlsPositive, negative, internal inhibition
Automatic AnalysisSupported with Open qPCR
Shipping TemperatureAmbient
Storage Temperature2 – 8 °C
ManufacturerPIKA Weihenstephan
Manufacturer Part #2401-41

Easy Workflow

Brewing Quality Solution Workflow
  • 20 minutes hands-on time.
  • Samples may be directly tested for 2 hour results.
  • Pre-enrichment with PCR-grade FastOrange increases sensitivity & degrades dead cells.


Product Manual Download
Material Safety Data Sheet (MSDS) Download