Megasphaera spoils beer by producing a putrid, fecal smell. Because it is an anaerobic bacteria extremeley sensitive to oxygen, it is difficult to detect by enrichment alone.
The PIKA 4e Megasphaera test kit screens for and quantifies all Megasphaera species. Includes all reagents needed to extract and amplify DNA using Real-Time PCR. 48 reactions/test kit.
|Sensitivity||10 CFU/mL (direct PCR)|
1 cell/sample (enriched)
|PCR Time||75 minutes|
|Compatible Samples||Beer, yeast slurry, colonies, environmental|
|Controls||Positive, negative, internal inhibition|
|Automatic Analysis||Supported with Open qPCR|
|Storage Temperature||2 – 8 °C|
|Manufacturer||PIKA Weihenstephan||Manufacturer Part #||2401-41|
- 20 minutes hands-on time.
- Samples may be directly tested for 2 hour results.
- Pre-enrichment with PCR-grade FastOrange increases sensitivity & degrades dead cells.