One-Step DNA/RNA Extraction Buffer
- Extracts both DNA and RNA in one simple step
- Lyses cells in minutes, without heat or mechanical agitation
- Large volumes of extract may be added directly to PCR reactions for sensitive detection
There's no need for any heating, centrifugation, or mechanical agitation steps for most samples. Simply expose cells to lysis buffer for 10 minutes at ambient temperature. Extraction efficiency for difficult-to-lyse cells can be improved by vortexing or homogenization.
The buffer is safe to handle; all reagents are non-toxic.
Lyse Liquid or Dry Samples
The One-Step DNA/RNA Extraction Buffer is available in two formats. The 1X concentration allows solid samples such as swabs, cell pellets, and membrane filters to be placed into buffer for lysis.
The 10X concentration can be added directly to liquid samples such as water, biological fluids, or transport media. This minimizes dilution of the sample, allowing sensitive detection by PCR.
Direct, Sensitive PCR
The One-Step DNA/RNA Extraction Buffer displays low PCR inhibition, allowing larger volumes of lysate to be added to PCR reactions for more sensitive detection. With our inhibition-tolerant PCR Master Mixes, up to 25% lysate may be used depending on sample type.
The One-Step DNA/RNA Extraction Buffer has been tested to work with:
|Bacteria (gram-positive, gram-negative)|
|Human cells (buccal, epithelial)|
|Viruses (RNA and DNA)|
DNA is a fairly stable molecule, but RNA notoriously degrades quickly. At 25 ºC, the One-Step DNA/RNA Extraction Buffer stabilizes DNA for up to 72 hours, and RNA for up to 24 hours, allowing flexibility in sample processing. DNA extracts can be stored long-term at -20 ºC, while RNA extracts can be stored at -70 ºC.
The buffer does not protect nucleic acid from DNase or RNase activity. For samples where this is a concern or for sensitive RNA extraction, we recommend our Enzymatic DNA/RNA Extraction Buffer.
|Lysis Temperature||20 – 30 ºC|
|DNA Extract Stability||72 hours at 25 ºC, long-term at -20 ºC|
|RNA Extract Stability||24 hours at 25 ºC, long-term at -70 ºC|
The One-Step DNA/RNA Extraction Buffer may be used license-free for commercial or diagnostic applications, and is available in bulk quantities for OEM use.
How does the buffer lyse cells?The One-Step DNA/RNA Extraction Buffer uses chemical means to disrupt cell membranes and stabilize DNA/RNA. No enzymes are employed, allowing the buffer to be stored long-term at ambient temperature.
Has this been validated for SARS-CoV-2?The buffer has been validated to lyse SARS-CoV-2 and is used in our Coronavirus Environmental Test Kit. However, due to the extremely rapid degradation of viral RNA by human RNases, it is not recommended for use with human specimens. We recommend our Enzymatic DNA/RNA Extraction Buffer for this purpose.
How does sensitivity compare with spin column purification?
Spin column purification has the advantage of concentrating the sample, but nucleic acid is lost at each purification step. While the direct PCR approach employed with the One-Step DNA/RNA Extraction Buffer does not concentrate the sample, it does eliminate the repeated losses of nucleic acid. Additionally, the One-Step DNA/RNA Extraction Buffer allows larger lysate volumes to be added to PCR reactions, aiding sensitivity.
Which approach is more sensitive for a given sample depends on the level of PCR inhibitors present and exact volumes of lysate added to the PCR reaction. The goal of the One-Step DNA/RNA Extraction Buffer is to provide Cq values reasonabily similar to spin column purification, but with a simpler and less costly workflow.
For Research Use Only. Not for use in diagnostic procedures.