Menu Chai Logo

Description


Chai Green™ is a new DNA binding dye optimized for Real-Time PCR and High Resolution Melt (HRM) analysis. It is significantly less inhibitory than SYBR Green because it binds primarily within the minor groove of DNA. Because of its lower inhibition, it may be used in higher concentrations resulting in increased fluorescence output, making it ideal for sensitive applications like HRM. The decreased PCR inhibition also decreases detection times in isothermal amplification applications.

Chai green manual cover @2x

Chai Green is compatible with all Real-Time PCR instruments supporting SYBR or FAM fluorophores, as its fluorescence spectrum is similar to SYBR Green. Dye binding appears to be cooperative and is influenced by the dye concentration and dye-to-basepair ratio.

Chai Green Fluorescence Spectrum
Chai Green Fluorescence Spectrum
The fluorescence spectrum of Chai Green™ is similar to that of SYBR Green.

Specifications


Concentration20X
Provided InWater
Storage Conditions

-20 °C

Protect from Light

Shipping TemperatureAmbient

Features


  • Thermally stable dye designed for qPCR, melt curve, and High Resolution Melt (HRM) analysis
  • Perform HRM at 1X working concentration without inhibiting PCR
  • No detectable loss of fluorescence after 20 freeze/thaw cycles
  • Produces lower detection times when used in isothermal amplification
  • Compatible with fast PCR protocols
  • No change of melt temperature when used at higher concentrations

License


Chai Green is an open license dye. You are free to use it for any use you like without any licensing restrictions or fees, and even to incorporate it within your own commercial products.

Performance


1X Chai Green Dye: 101% Amplification Efficiency
GAPDH Standard Curve Amplified With Chai 2X PCR Master Mix + Hot Start
Five ten-fold serial dilutions of a GAPDH gene fragment were used to create a PCR standard curve. A 123 bp product was amplified using 1X Chai Green concentration and Chai's 2X PCR Master Mix + Hot Start. An efficiency of 101% was obtained, with a slope of -3.328 and R2 value of 1.000.

Chai Green Fluorescence Compared With SYBR Green I
Chai Green Fluorescence Compared With SYBR Green I
1X Chai Green (red) compared with the highest concentration of SYBR Green I (black) that did not exhibit PCR inhibition. Chai Green is significantly more fluorescent, leading to improved melt curve & HRM performance.

Protocol


Store Chai Green at -20 °C and protect from light. Chai Green is provided as a 20X solution in water. Use the dye at a 1X concentration in the qPCR reaction. Add 1.25 μL 20X Chai Green per 25 μL reaction. Use with a thermocycler capable of measuring SYBR Green I or FAM fluorescence during the extension phase of qPCR. The 1X working concentration may also be used for melt curve analysis and High Resolution Melt (HRM).

Downloads


Product Manual Download
Safety Data Sheet (SDS) Download