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Chai Open qPCR




Used by hundreds of customers
in research, academia, biotech, brewing, and more.

Single Channel $3,749

Dual Channel $5,499

Open qPCR Real Time PCR machine
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Advanced tech that delivers reliable, accurate and precise test results

Open qPCR delivers thermal control so precise that results can be directly compared across runs, eliminating the need to repeatedly generate standard curves.

Open qPCR Amplification plot

Amplification plot for 16 replicates of an assay with FAM-labeled probes.

Product accurate, consistent results every time with a photodiode-based, solid-state design, precision temperature control, and advanced software.

Open qPCR Optics module

Setup instrument, run advanced experiments and analyze test results with ease

Get up and running fast with simple, do-it-yourself setup and calibration. Our web-based applicatio makes it easy to design even the most complex experiments.

Open qPCR Setup Experiment

Protocol Design Screen

Our dynamic Edit Protocol screen lets users of any skill level design even the most advanced experiments.

Run a full range of experiments:
  • Presence/absence
  • Relative quantification
  • Absolute quantification
  • Melt curve
  • High resolution melt
  • Genotyping / Allelic discrimination plot
Including these optional features:
  • Reverse transcription (RT-PCR) (a big one)
  • Autodelta, or Touch-down PCR
  • Controlled ramp rates

Our Open API allows integration with custom analytical or laboratory automation systems.

Insanely fast: Run more experiments in less time

Our 16-well analyzes up to 16 different samples at once. Do even more in less time with our Dual Channel model, analyzing up to 32 targets.

A dual-peltier design and a heated lid deliver temperature ramp rates of 5 °C/s. Get your results in as little as 20 minutes.

Open qPCR cycles

Works wherever you are. And where you're not.

Open qPCR Front, Back and Side

Open qPCR has small footprint(11" x 9.5"), so you can use it anywhere you have power. And at only 10 lbs, it's easy to ship.

On-site or remote, you and your team can run and analyze experiments using their favorite browser.

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Take your investment further with Chai's quality reagents

Chai PCR Master Mix Reagent

Our reagents and consumables deliver excellent performance at affordable prices.

Open qPCR is compatible with all Chai products and outside manufacturer's master mixes.

Industries and applications

Open qPCR detects pathogens, genetic mutations, and dangerous diseases using DNA diagnostics. It is used in a wide range of applications by researchers, educators, industrial microbiologists, forensic experts, biohackers and more.

Uses are endless and include:
  • Biomedical research for assay optimization
  • Gene expression analysis
  • Protein analysis
  • Pathogen identification
  • ...and many more
Device of choice for industrial use like:
  • Bacterial and pathogen identification
  • Water quality
  • Food testing
  • Beer and wine quality
  • Cannabis mold content



Samples: 16 wells, 100 µL low profile tubes
Sample Volume: 10 – 50 µL
Sensitivity: 1 copy
Dynamic Range 109
Dimensions: 11 x 9.5 x 7.5 inches (28 x 24 x 19 cm)
Weight: 9 lbs (4 kg)
Approvals: FCC, CE


Temp Range: 4 – 100 °C
Ramp Rate: 0.00001 – 5.0 °C/s
Design Dual Peltiers
Temp Accuracy: ±0.2 °C at 65 °C
Temp Uniformity: ±0.4 °C at 65 °C
Lid Temp: Ambient – 120 °C


Excitation: 454 – 487 nm
Detection (Single Ch): 513 – 555 nm
Detection (Dual Ch): 508 – 532, 573 – 597 nm
Fluorophores: Chai Green, SYBR Green, FAM, HEX, VIC, JOE
Scan Time: 4.4 s
Design: Solid-State LEDs & Photodiodes


Networking: Ethernet, 802.11n WiFi, USB
Touchscreen: 5.0 inch, Capacitive
Software: HTML5/JavaScript Web App
Device Support: PC, Mac, Linux, iPad, Android Tablet
Integration: REST API


Voltage: 100 – 240 V
Frequency: 50 – 60 Hz
Avg Power: 75 W
Peak Power: 350 W*
* Peak power can be limited to 125 W by limiting ramp speed to 1 °C/s for battery operation

Operating Environment

Temperature: 10 – 35 °C
Humidity: 20 – 80% non-condensing
Max Altitude: 2,000 m

Open source licenses

Software: Apache v2
Hardware: Creative Commons Attribution

Analysis Features

Baseline Subtraction Melt Curve Analysis
Color Compensation High Resolution Melt
Data Export



Open qPCR User Manual Download
FCC Verification of Conformity Download