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A FAST, ACCURATE, AND PORTABLE REAL-TIME PCR MACHINE

Used by thousands of customers
in research, academia, biotech, brewing, and more.

Single Channel $4,299

Dual Channel $5,999



UCSF Yale Oxford CDC NIH USDA

Precise, repeatable, and accurate qPCR results

Open qPCR's thermal control is so exact that results can be directly compared across runs, eliminating the need to generate standard curves repeatedly.

Open qPCR produces Real-Time PCR amplification curves with tight qPCR replicate results

Amplification plot for 16 replicates of an assay with FAM-labeled probes.

The instrument produces accurate, consistent results every time with a photodiode-based solid-state design, precision temperature control, and advanced software.

Open qPCR's fluorescence detection optics are solid-state

Setup instrument, run advanced experiments and analyze test results with ease

Get up and running fast with simple, do-it-yourself setup and calibration. Our web-based application makes it easy to design even the most complex experiments.

Open qPCR machine's visual protocol editor allows advanced Real-Time PCR experiments to be easily defined

Protocol editor lets novice users design even the most advanced experiments.


Run a full range of experiments:
  • Presence/absence
  • Relative quantification
  • Absolute quantification
  • Melt curve
  • High resolution melt
  • Genotyping / Allelic discrimination plot
  • Reverse transcription (RT-PCR)
Advanced features:
  • Autodelta, or Touch-down PCR
  • Controlled ramp rates
  • Multiplexing (requires dual channel)

Our Open API allows integration with custom analytical or laboratory automation systems.


Insanely fast: Run PCR In 20 Minutes

With both heating and cooling rates of 5 °C/s, Open qPCR can run 40 cycles of PCR in 20 minutes, allowing you to get results faster and run more experiments.

Open qPCR machine runs 40 cycles of PCR in 20 minutes

Run on your bench, view results at your desk

Open qPCR is a small, compact, rugged Real-Time PCR machine

The Open qPCR machine has a small footprint (11" x 9.5" or 28 cm x 24 cm), so you can use it anywhere you have power. And at only 9 lbs (4 kgs), it's easy to ship.

On-site or remote, you and your team can run and analyze experiments using their favorite browser on a computer, tablet or smartphone.

USB WiFi Ethernet Ethernet

Take your investment further with Chai's quality reagents

PCR Master Mix

Our master mixes and consumables deliver excellent performance at affordable prices.

Open qPCR is compatible with all Chai products and other vendor's master mixes.


Industries and applications

Open qPCR detects pathogens, genetic mutations, and dangerous diseases using DNA diagnostics. It is used in a wide range of applications by researchers, educators, industrial microbiologists, forensic experts, biohackers and more.

Uses are endless and include:
  • Assay optimization for biomedical research
  • Gene expression analysis
  • Pathogen identification
  • Library preparation for next-gen sequencing
  • and many more
Also, its a device of choice for industrial uses like:
  • Bacterial and pathogen identification
  • Water quality
  • Food testing
  • Beer spoilage
  • Cannabis mold content

 

 


Research citations

Damase TR, Miura TA, Parent CE, Allen PB. 2018. Application of the Open qPCR Instrument for the in Vitro Selection of DNA Aptamers against Epidermal Growth Factor Receptor and Drosophila C Virus. ACS Comb Sci. 20(2):45–54.

Taylor BJ, Lanke K, Banman SL, Morlais I, Morin MJ, Bousema T, Rijpma SR, Yanow SK. 2017. A Direct from Blood Reverse Transcriptase Polymerase Chain Reaction Assay for Monitoring Falciparum Malaria Parasite Transmission in Elimination Settings. Am J Trop Med Hyg. 97(2):533-543.

TECH SPECS

PCR

Samples: 16 wells, 100 µL low profile tubes
Supported Probes: TaqMan, Molecular Beacon, Scorpions, Hydrolysis
Supported Dyes: Chai Green, SYBR Green, EvaGreen, LCGreen
Supported Fluorophores: FAM, HEX, VIC, JOE
Sample Volume: 10 – 50 µL
Sensitivity: 1 copy
Dynamic Range 109

Optical

Excitation: 454 – 487 nm
Detection (Single Ch): 513 – 555 nm
Detection (Dual Ch): 508 – 532, 573 – 597 nm
Scan Time: 4.4 s
Design: Solid-State LEDs & Photodiodes

Thermal

Temp Range: 4 – 100 °C
Ramp Rate: 0.00001 – 5.0 °C/s
Temp Accuracy: ±0.2 °C at 65 °C
Temp Uniformity: ±0.4 °C at 65 °C
Lid Temp: Ambient – 120 °C

Connectivity

Networking: Ethernet, 802.11n WiFi, USB
Software: Web Application
Device Support: PC, Mac, Linux, iPad, Android Tablet
Integration: REST API

SYSTEM

Power: 100 – 240 V, 50 – 60 Hz
Operating Temp: 10 – 35 °C
Operating Humidity: 20 – 80% non-condensing
Dimensions: 28 x 24 x 19 cm (11 x 9.5 x 7.5 inches)
Weight: 4 kg (9 lbs)
Approvals: FCC, CE

DOWNLOADS

RESOURCES

Open qPCR User Manual PDF
FCC Verification of Conformity PDF
CE Declaration of Conformity PDF