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Chai Open qPCR




Used by hundreds of customers
in research, academia, biotech, brewing, and more.

Single Channel $4,299

Dual Channel $5,999

Open qPCR Real Time PCR machine
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An advanced qPCR machine that delivers reliable, accurate and precise test results

Open qPCR's thermal control is so exact that results can be directly compared across runs, eliminating the need to generate standard curves repeatedly.

Open qPCR Amplification plot

Amplification plot for 16 replicates of an assay with FAM-labeled probes.

The instrument produces accurate, consistent results every time with a photodiode-based solid-state design, precision temperature control, and advanced software.

Open qPCR Optics module

Setup instrument, run advanced experiments and analyze test results with ease

Get up and running fast with simple, do-it-yourself setup and calibration. Our web-based application makes it easy to design even the most complex experiments.

Open qPCR Setup Experiment

Protocol editor lets novice users design even the most advanced experiments.

Run a full range of experiments:
  • Presence/absence
  • Relative quantification
  • Absolute quantification
  • Melt curve
  • High resolution melt
  • Genotyping / Allelic discrimination plot
  • Reverse transcription (RT-PCR)
Advanced features:
  • Autodelta, or Touch-down PCR
  • Controlled ramp rates
  • Multiplexing (requires dual channel)

Our Open API allows integration with custom analytical or laboratory automation systems.

Insanely fast: Run PCR In 20 Minutes

With both heating and cooling rates of 5 °C/s, Open qPCR can run 40 cycles of PCR in 20 minutes, allowing you to get results faster and run more experiments.

Open qPCR cycles

Run on your bench, view results at your desk

Open qPCR Front, Back and Side

Open qPCR has a small footprint (11" x 9.5" or 28 cm x 24 cm), so you can use it anywhere you have power. And at only 9 lbs (4 kgs), it's easy to ship.

On-site or remote, you and your team can run and analyze experiments using their favorite browser on a computer, tablet or smartphone.

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Take your investment further with Chai's quality reagents

Chai PCR Master Mix Reagent

Our master mixes and consumables deliver excellent performance at affordable prices.

Open qPCR is compatible with all Chai products and other vendor's master mixes.

Industries and applications

Open qPCR detects pathogens, genetic mutations, and dangerous diseases using DNA diagnostics. It is used in a wide range of applications by researchers, educators, industrial microbiologists, forensic experts, biohackers and more.

Uses are endless and include:
  • Assay optimization for biomedical research
  • Gene expression analysis
  • Pathogen identification
  • Library preparation for next-gen sequencing
  • and many more
Also, its a device of choice for industrial uses like:
  • Bacterial and pathogen identification
  • Water quality
  • Food testing
  • Beer and wine quality
  • Cannabis mold content



Samples: 16 wells, 100 µL low profile tubes
Sample Volume: 10 – 50 µL
Sensitivity: 1 copy
Dynamic Range 109
Dimensions: 11 x 9.5 x 7.5 inches (28 x 24 x 19 cm)
Weight: 9 lbs (4 kg)
Approvals: FCC, CE


Temp Range: 4 – 100 °C
Ramp Rate: 0.00001 – 5.0 °C/s
Design Dual Peltiers
Temp Accuracy: ±0.2 °C at 65 °C
Temp Uniformity: ±0.4 °C at 65 °C
Lid Temp: Ambient – 120 °C


Excitation: 454 – 487 nm
Detection (Single Ch): 513 – 555 nm
Detection (Dual Ch): 508 – 532, 573 – 597 nm
Fluorophores: Chai Green, SYBR Green, FAM, HEX, VIC, JOE
Scan Time: 4.4 s
Design: Solid-State LEDs & Photodiodes


Networking: Ethernet, 802.11n WiFi, USB
Touchscreen: 5.0 inch, Capacitive
Software: HTML5/JavaScript Web App
Device Support: PC, Mac, Linux, iPad, Android Tablet
Integration: REST API


Voltage: 100 – 240 V
Frequency: 50 – 60 Hz
Avg Power: 75 W
Peak Power: 350 W*
* Peak power can be limited to 125 W by limiting ramp speed to 1 °C/s for battery operation

Operating Environment

Temperature: 10 – 35 °C
Humidity: 20 – 80% non-condensing
Max Altitude: 2,000 m

Open source licenses

Software: Apache v2
Hardware: Creative Commons Attribution

Analysis Features

Baseline Subtraction Melt Curve Analysis
Color Compensation High Resolution Melt
Data Export



Open qPCR User Manual PDF
FCC Verification of Conformity PDF
CE Declaration of Conformity PDF