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Your Personal
Real-Time PCR Machine

Fast, Accurate, and Portable

Single Channel $4,499

Dual Channel $6,499


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qPCR Machine
UCSFYaleOxfordCDCNIHUSDA
Real-Time PCR Amplification Curves

Precise, Consistent qPCR Results

Ultra-tight replicates. Results so repeatable they can be compared across runs. Quantification so accurate that two-fold differences in gene expression can be detected.

Explore Performance
Real-Time PCR Amplification Curves
qPCR Machine Software

Powerful, User-Friendly Software

Rapidly design, run, and analyze advanced experiments.

Browser-based software is always up to date. There’s nothing to install: Open qPCR’s software runs right in your web browser on any Mac, PC, or tablet.

Explore Software

Compact, Portable, Connected

Start a run at the lab, view results from your home, or anywhere you have Internet.

Or take Open qPCR into the field. Built like a tiny tank and weighing only 4 kg, it’s easy to transport, and can run anywhere you have power.

  • Usb
  • Wifi
  • Ether
qPCR Machine Rear
qPCR Machine Side

Insanely Fast

Get rapid results for rapid decision making. Open qPCR can run 40 cycles of PCR in 20 minutes, making it the device of choice for time-sensitive applications.

Fast Real-Time PCR Machine does 40 cycles in 20 minutes

Open Platform


Avoid vendor lock-in. Open qPCR works with all PCR master mixes, probe chemistries, and dyes.

Open Source


We promote research & support the community by making our core bioinformatics algorithms open source.

Open API


Control Open qPCR via your laboratory automation system or custom software with our open API.

Tech Specs

PCR

Samples:
Supported Probes:
TaqMan, Molecular Beacon, Scorpions, Hydrolysis
Supported Dyes:
Chai Green, SYBR Green, EvaGreen, LCGreen
Supported Fluorophores:
FAM, HEX, VIC, JOE
Sample Volume:
10 – 50 µL
Sensitivity:
1 copy
Dynamic Range
1010

Optical

Excitation:
454 – 487 nm
Detection (Single Ch):
513 – 555 nm
Detection (Dual Ch):
508 – 532, 573 – 597 nm
Scan Time:
4.4 s
Design:
Solid-State LEDs & Photodiodes

Thermal

Temp Range:
4 – 100 °C
Ramp Rate:
0.00001 – 5.0 °C/s
Temp Accuracy:
±0.2 °C at 65 °C
Temp Uniformity:
±0.4 °C at 65 °C
Lid Temp:
Ambient – 120 °C

Connectivity

Networking:
Ethernet, 802.11n WiFi, USB
Software:
Web Application
Device Support:
Mac, PC, Linux, iPad, Android Tablet
Integration:
REST API

System

Power:
100 – 240 V, 50 – 60 Hz
Operating Temp:
10 – 35 °C
Operating Humidity:
20 – 80%, non-condensing
Dimensions:
28 x 24 x 19 cm  (11 x 9.5 x 7.5 inches)
Weight:
4 kg  (9 lbs)
Approvals:
FCC, CE

Research Citations

Taylor BJ, Lanke K, Banman SL, Morlais I, Morin MJ, Bousema T, Rijpma SR, Yanow SK. 2017. A Direct from Blood Reverse Transcriptase Polymerase Chain Reaction Assay for Monitoring Falciparum Malaria Parasite Transmission in Elimination Settings. Am J Trop Med Hyg. 97(2):533-543.

Damase TR, Miura TA, Parent CE, Allen PB. 2018. Application of the Open qPCR Instrument for the in Vitro Selection of DNA Aptamers against Epidermal Growth Factor Receptor and Drosophila C Virus. ACS Comb Sci. 20(2):45–54.

Damse TR, Allen PB. 2019. Idiosyncrasies of thermofluorimetric aptamer binding assays. BioTechniques. 66(5).

Aufdembrink LM, Hoog TG, Pawlak MR, Bachan BF, Heili JM, Engelhart AE. 2019. Methods for thermal denaturation studies of nucleic acids in complex with fluorogenic dyes. In: Hargrove AE, editor. Methods in Enzymology. Volume 623. Cambridge (MA): Elsevier. p. 23-43.


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Sahara Multiplex qPCR Master Mix
Sahara Multiplex qPCR Master Mix

Multiplex up to 4 targets with high efficiency and consistent Cq's. Exceptionally thermal stable mix resists inhibition, even in environmental samples. For multiplex probe-based qPCR.

Chai Green qPCR Master Mix
Chai Green qPCR Master Mix

Get clear melt curves and High Resolution Melt (HRM) results with the highly fluorescent Chai Greey dye. Highly efficient mix resists inhibition, even in environmental samples. For dye-based qPCR.

PCR Tube & Cap Strips, 8-Well Strips, 100 µL, Optically Clear
PCR Tube & Cap Strips, 8-Well Strips, 0.1 mL

Thin-walled, optically-clear PCR tube & cap strips. DNase, RNase, and PCR-inhibitor free. 125 8-well tube+cap strips/box.


For research use only. Open qPCR machine not for use in diagnostic procedures.