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Sahara One-Step RT-qPCR Master Mix

Sahara One-Step RT-qPCR Master Mix

$0.00


Highlights


  • Multiplex up to four RT-qPCR reactions with no loss of efficiency
  • Thermally stable mix withstands 40 ºC for seven days without loss of performance
  • Fast 5 minute reverse transcription step
  • Overcomes inhibition in crude extracts and environmental samples
  • License-free for commercial or diagnostic use

Description


Sahara One-Step RT-qPCR Master Mix is a high performance mix for multiplex, probe-based quantitativie reverse transcription PCR (RT-qPCR). It amplifies RNA targets at high efficiency while resisting PCR inhibitors. The one-step mix allows cDNA synthesis and subsequent PCR amplification to be performed in a single reaction, maximizing operational efficiency and sensitivity.

Extreme Thermal Stability

Stable at 40 ºC for seven days, or 25 ºC for weeks, Sahara One-Step RT-qPCR Master Mix ships economically at ambient temperatures, prevents Cq drift over time due to thermal degradation, and eases reaction setup logistics for both humans and robots.

Fast PCR and Reverse Transcription

The fast reaction mix requires only 5 minutes for reverse transcription, and can subsequently perform 40 cycles of amplification in 20 minutes.

Amplifies Everything Without Inhibition

Sahara One-Step RT-qPCR Master Mix has been validated against mRNA, rRNA, viral RNA, and genomic DNA targets from a wide range of organisms. Numerous enhancers bind & inactivate PCR inhibitors, allowing amplification from crude extracts. The mix amplifies GC-rich targets up to 70%

Safeguard Against Carry-over Contamination

The mix contains a 90% dUTP/dTTP ratio, allowing amplicons to be selectively degraded. If you later encounter carry-over contamination resulting in false positives/amplification of NTC reactions, switching to the Sahara One-Step RT-qPCR Master Mix with UNG will degrade carry-over contamination.

Specifications


Concentration2X
Optimal RT Activity Temp60 – 65 ºC
RT Step Duration5 min
Hot Start MechanismAptamer
Carry-over PreventionCompatible, dUTP included
GC ContentUp to 70%
MultiplexingUp to 4-plex
Reaction SpeedFast
RNase InhibitorYes
Visible Loading DyeYes
Exonuclease Activity5' → 3'
Supported ProbesTaqMan/Hydrolysis, Molecular Beacon, Scorpions
Supported TemplatesTotal RNA, mRNA, rRNA, viral RNA, DNA
Recommended Reaction Volume10 – 50 µL

Mix is provided with a 9:1 dUTP/dTTP ratio, allowing amplified fragments to be degraded with Sahara One-Step RT-qPCR Master Mix with UNG.

Reaction Conditions
dNTPs0.5 mM dATP/dCTP/dGTP
0.45 mM dUTP
0.05 mM dTTP
K+20 mM
Mg2+4 mM
Mn2+0.75 mM, adjustable 0.5 – 1.5 mM
Shipping and Storage
Temperature Stability7 days @ 40 ºC, weeks @ 25 ºC
Freeze/Thaw CyclesUp to 20
Shipping ConditionsAmbient
Storage Conditions-20 ºC, or 4 ºC for 6 months. Protect from light.
Compatible Instruments

The mix ships with an optional ROX reference dye, allowing it to be used with high, low, and no-ROX qPCR instruments. 

FAQs


  • What is a PCR Master Mix?
    A PCR master mix is a premixed solution that contains most of the components necessary to run a PCR assay. The mix contains Taq DNA polymerase, dNTPs, MgCl2, as well as enhancers and stabilizers in a buffer that is optimized for DNA amplification by PCR. Read more...
View all related knowledge-base articles.
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License


To promote collaboration in science, Sahara One-Step RT-qPCR Master Mix is provided under an open license:

  • It may be used freely for any application, including commercial and diagnostic use
  • No license agreements or royalty payments required for OEM use
  • May be incorporated into your own test kits without restriction

Resources


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