Sahara Universal qPCR Master Mix
- Multiplex two Real-Time PCR reactions with no effect on Cq’s
- Extreme thermal stability: withstands three months at 25 ºC with no impact on efficiency
- Overcomes inhibition in crude extracts and environmental samples
- Universal mix runs fast protocols, amplifies GC-rich targets, and incorporates aptamer-based hot start and dUTP carry-over prevention systems
- License-free for commercial or diagnostic use
Sahara Universal qPCR Master Mix is a 2X Taq mix for probe-based Real-Time PCR. It quantitatively amplifies qPCR targets at high efficiency from purified samples and crude extracts.
The Most Stable Master Mix on the Planet
Stable at 50 ºC for eight days, or 25 ºC for three months, Sahara mixes ship economically at ambient temperatures, prevent Cq drift over time due to thermal degradation, and are ideal for high throughput or field applications where temperature control is not always ideal.
Amplifies Everything Without Inhibition
Sahara Universal qPCR Master Mix has been validated against genomic, plasmid, and cDNA targets from a wide range of organisms. The mix supports both standard and fast protocols, and amplifies GC-rich targets up to 70%.
Numerous enhancers bind & inactivate PCR inhibitors, allowing amplification from crude extracts of:
- Food & beverages
- Plant & animal tissue
- Environmental air & water samples
Simplify your PCR
Set up reactions with ease at room temperature: an aptamer-based hotstart system prevents amplification from occurring below 50 ºC, while a blue non-interfering loading dye helps avoid pipetting errors. When activated by the addition of UNG, a dUTP carry-over prevention system prevents false positives from amplicon contamination.
Excellent efficiency and linearity using Sahara Universal qPCR Master Mix to amplify a 157 bp gene fragment on an Open qPCR instrument.
High Efficiency Duplexing
E = 99.2%, R2 = 1.000
E = 99.6%, R2 = 0.999
Two targets were amplified simultaneously using Sahara Universal qPCR Master Mix on a Bio-Rad CFX Touch instrument. 10-fold dilutions of all targets achieved excellent linearity & efficiency.
For multiplexing up to four targets simultaneously, please see our Sahara Multiplex qPCR Master Mix.
Sahara Universal qPCR Master Mix was stored at 50 ºC, and duplex reactions were run daily on Bio-Rad CFX96 Touch instrument. Consistent Cq values were observed throughout the trial.
Fast & Quantitative
A fast 19 minute protocol was run on an Open qPCR instrument, obtaining excellent linearity & efficiency. Protocol: Initial denaturing 30s @ 95 ºC, Cycle 30x: 3s @ 95 ºC, 14s @ 60 ºC, 5 C ºC/s ramp times.
|Hot Start Mechanism||Aptamer|
|GC Content||40 – 70%|
|Multiplex Targets||Up to 2|
|dUTP Carryover Prevention||Yes†|
|Visible Loading Dye||Yes|
|Exonuclease Activity||5' → 3'|
|Supported Probes||TaqMan/Hydrolysis, Molecular Beacon, Scorpions|
|Supported Templates||Genomic DNA, cDNA, Plasmid DNA|
|Recommended Reaction Volume||10 – 50 µL|
† user must add UNG to enable
Shipping, Storage, and Stability
|High Temperature Stability||8 days @ 50 ºC, 3 months @ 25 ºC|
|Freeze/Thaw Cycles||Up to 20|
|Storage Conditions||-20 ºC long-term, or 4 ºC for 6 months|
The mix ships with an optional ROX reference dye, allowing it to be used with high, low, and no-ROX qPCR instruments.
What is a PCR Master Mix?A PCR master mix is a premixed solution that contains most of the components necessary to run a PCR assay. The mix contains Taq DNA polymerase, dNTPs, MgCl2, as well as enhancers and stabilizers in a buffer that is optimized for DNA amplification by PCR. Read more...
To promote collaboration in science, Sahara Universal qPCR Master Mix is provided under an open license:
- It may be used freely for any application, including commercial and diagnostic use
- No license agreements or royalty payments required for OEM use
- May be incorporated into your own test kits without restriction