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PCR Master Mixes

For routine and Real-Time PCR

PCR Master Mix PCR Master Mix
+ Hot Start
Chai Green PCR
Master Mix + Hot Start
Ideal For Routine PCR Real-Time PCR
Robust PCR
Real-Time PCR
High Resolution Melt (HRM)
Detection Chemistry Gels Dyes, Probes Chai Green Dye
Enhanced Specificity No Yes Yes
Activation Time Immediate 30 s - 2 min 30 s - 2 min
Benefits Low cost
High yield
Prevent Primer Dimers High efficiency Prevent Primer Dimers
High efficiency
Low cost qPCR
High Resolution Melt (HRM)

Chai Master Mixes are optimized ready-to-use solutions containing Taq polymerase, dNTPs, buffers, cofactors, enhancers, and stabilizers. Just add template DNA, primers, and optionally probes, and you’re ready to go. All master mixes have been validated for amplicon lengths of up to 5 kb.

Hot Start mixes add an additional layer of PCR specificity through the inactivation of Taq polymerase at lower temperatures. Following the initial denaturation step, the polymerase is activated and amplification proceeds with greater specificity.

  • Pcr master mix

    PCR Master Mix 2X

    Catalog #: R02100

    High-yield Taq PCR master mix for routine PCR. Amplifies products up to 5 kb.
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  • Pcr master mix hot start

    PCR Master Mix with Hot Start 2X

    Catalog #: R02150

    High efficiency Taq-based master mix ideal for Real-Time PCR. Aptamer-based Hot Start technology enhances specificity and requires only 30 seconds to activate. Compatible with probe chemistries.
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  • Chai green pcr master mix

    Chai Green Master Mix + Hot Start 2X

    Catalog #: R02200

    High efficiency Real-Time PCR master mix with Chai Green dye ideal for singleplex qPCR and HRM applications. Aptamer-based Hot Start technology enhances specificity and requires only 30 seconds to activate.
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