Quantify DNA & RNA with confidence. Open qPCR uses advanced Cy0-based bioinformatics to make precision Cq calls.
Shown: Ara h2 gene fragment 5-point standard curve, amplified with Sahara Hot Start PCR Master Mix. Efficiency = 98.8%, R² = 1.000
Wide Dynamic Range
Detect a singly copy or ten billion in the same experiment. Open qPCR provides exceptional linearity over a 1010 dynamic range.
Shown: 10 point, 10-fold dilution series of Ara h2 gene fragment using Sahara Multiplex qPCR Master Mix. Efficiency = 99.96%, R² = 1.000
Detect Even Two-Fold Differences
Open qPCR’s solid-state optics are so sensitive that even two-fold differences in gene expression levels can be differentiated.
Shown: Lambda DNA 2x dilution series in triplicate with 200 bp target amplified with Sahara Hot Start PCR Master Mix. Efficiency = 105%, R² = 0.999.
Ultra Tight Replicates Every Time
Exceptional temperature uniformity and control produce consistent results across all wells.
Samples are repeatable and can even be compared across runs. Run standard curves once and reuse as needed.
Shown: 16 HPRT gene fragment replicates amplified with Sahara Hot Start PCR Master Mix. Avg Cq = 21.6, Standard Deviation = 0.03
Results in 20 Minutes
Run fast 30 cycle protocols providing quantitative results in 20 minutes, or run 40 cycles in 20 minutes for presence/absence assays.
Shown: Lambda DNA 5-point, 10-fold dilution using Chai Green qPCR Master Mix. 30 cycles, 19 minute run-time. Efficiency = 106%, R² = 0.996
Distinguish different genotypes and PCR products with ease thanks to Open qPCR’s thermal uniformity of ±0.25°C.
Shown: HPRT gene fragment replicates (n = 16) using Chai Green qPCR Master Mix. Tm uniformity = ± 0.22 ºC
Multiplex Without Crosstalk
Detect two targets per sample with our dual channel device. Open qPCR uses dedicated per-channel photodetectors and automatically applied color compensation/deconvolution to virtually eliminate crosstalk between channels.
Shown: mGAPDH (Ch 1) and Lambda DNA (Ch 2) targets of differing quantities, run with Sahara Multiplex qPCR Master Mix.